Anti-CXCL12 Antibody, Rabbit, Polyclonal

Catalog Number: BOB-A00053-2-CARRIER-FREE
Article Name: Anti-CXCL12 Antibody, Rabbit, Polyclonal
Biozol Catalog Number: BOB-A00053-2-CARRIER-FREE
Supplier Catalog Number: A00053-2-carrier-free
Alternative Catalog Number: BOB-A00053-2-CARRIER-FREE-100UG
Manufacturer: Boster Bio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC
Species Reactivity: Human, Mouse, Rat
Immunogen: E. coli-derived human CXCL12 recombinant protein (Position: V24-M93).
Alternative Names: C X C motif chemokine 12, CXCL12, CXCL12/SDF-1, hIRH, hSDF 1, IRH, PBSF, SCYB12, SDF 1, SDF 1a, SDF 1b, SDF1, SDF-1, SDF1A, SDF1B, Stromal cell derived factor 1, TLSF a, TLSF b, TPAR1
Boster Bio Anti-CXCL12 Antibody Picoband catalog A00053-2. Tested in ELISA, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Clonality: Polyclonal
Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Molecular Weight: Observed Molecular Weight: 11 kDa. Calculated Molecular Weight: 25283 MW
NCBI: 6387
UniProt: P48061
Buffer: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Purity: Immunogen affinity purified.
Form: Lyophilized
Target: Stromal cell-derived factor 1
Application Dilute: Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml Immunofluorescence, 5 µg/ml ELISA, 0.1-0.5µg/ml
IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). CXCL12 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IF analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). CXCL12 was detected in a paraffin-embedded section of human hepatitis tissue. Heat mediated antigen retrie
IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).CXCL12 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).CXCL12 was detected in paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). CXCL12 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). CXCL12 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.
IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).CXCL12 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.