Anti-CXCL12 Antibody Cy3 Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Anti-CXCL12 Antibody Cy3 Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: BOB-A00053-2-CY3
• Supplier Catalog Number: A00053-2-Cy3
• Alternative Catalog Number: BOB-A00053-2-CY3-100UG
• Manufacturer: Boster Bio
• Host: Rabbit
• Category: Antikörper
• Application: FC
• Species Reactivity: Human, Mouse, Rat
• Immunogen: E. coli-derived human CXCL12 recombinant protein (Position: V24-M93).
• Alternative Names: C X C motif chemokine 12, CXCL12, CXCL12/SDF-1, hIRH, hSDF 1, IRH, PBSF, SCYB12, SDF 1, SDF 1a, SDF 1b, SDF1, SDF-1, SDF1A, SDF1B, Stromal cell derived factor 1, TLSF a, TLSF b, TPAR1

Product Properties

• Clonality: Polyclonal
• Molecular Weight: Calculated Molecular Weight: 25283 MW
• NCBI: 6387
• UniProt: P48061
• Buffer: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
• Purity: Immunogen affinity purified.
• Form: Liquid
• Target: Stromal cell-derived factor 1

Application Notes

• Application Dilute: Flow Cytometry, 1-3µg/1x106 cells

Images

IF analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). CXCL12 was detected in a paraffin-embedded section of human hepatitis tissue. Heat mediated antigen retrie

IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).CXCL12 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.

IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).CXCL12 was detected in paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.

IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). CXCL12 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.

IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). CXCL12 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1022) with DAB as the chromogen.

IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).CXCL12 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.

IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). CXCL12 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.