E.coli-derived human ALKBH5 recombinant protein (Position: E81-R180).
Alternative Names:
ABH5, ALKBH5, OFOXD, OFOXD1
Boster Bio Anti-ALKBH5 Antibody Picoband catalog A03360. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Purity:
Immunogen affinity purified.
Form:
Lyophilized
Target:
RNA demethylase ALKBH5
Application Dilute:
Western blot, 0.1-0.25µg/ml, Human Immunocytochemistry/Immunofluorescence, 5µg/ml, Human ELISA, 0.1-0.5µg/ml, -
Transfection validation of ALKBH5 overexpression and interference ( A . IHH4 cells, B . TPC-1 cells). Index in PubMed under a CC BY license. PMID: 39789120
Proliferation and invasion of IHH4 and TPC-1 Cells Detected by CCK-8 Assay and Transwell Assay ( A . CCK-8 assay to detect the cell viability of IHH4 and TPC-1 cells, B . Transwell assay to assess the invasion of IHH4 cells, C . Transwell assay to assess the invasion of TPC-1 cells. * P <0.05 indicates a significant difference between each group compared to the OE-NC group, P <0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group, & P <0.05 indicates a significant difference between each group compared to the Si-NC group). Index in PubMed under a CC BY license. PMID: 39789120
Proliferation and invasion of IHH4 and TPC-1 Cells Detected by CCK-8 Assay and Transwell Assay ( A . CCK-8 assay to detect the cell viability of IHH4 and TPC-1 cells, B . Transwe
IF analysis of ALKBH5 using anti-ALKBH5 antibody (A03360) and anti-Tubulin Alpha antibody (M03989-3).ALKBH5 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-ALKBH5 Antibody (A03360) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Western blot analysis of ALKBH5 using anti-ALKBH5 antibody (A03360). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALKBH5 antigen affinity purified polyclonal antibody (Catalog A03360) at 0.25 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for ALKBH5 at approximately 52 kDa. The expected band size for ALKBH5 is at 44 kDa.
Comparison of human samples through immunohistochemical detection of ALKBH5 expression. ( * P <0.05 indicates a significant difference between the indicated groups). Index in PubMed under a CC BY license. PMID: 39789120
( A ) qRT-PCR and Western blot were used to detect the expression of ALKBH5 to screen PTC cells. ( * P <0.05 indicates a significant difference between each group compared to the Nthy-ori3-1 group). ( B ) Subcellular Localization of ALKBH5 Protein Detected by Immunofluorescence. Index in PubMed under a CC BY license. PMID: 39789120
Transfection validation of ALKBH5 overexpression and interference ( A . IHH4 cells, B . TPC-1 cells). Index in PubMed under a CC BY license. PMID: 39789120
* VAT and and shipping costs not included. Errors and price changes excepted
