0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Purity:
Anti-Plasminogen is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Plasminogen [Human Plasma]. Cross-reactivity against Plasminogen from other sources is unknown.
Form:
Lyophilized
Application Dilute:
ELISA: 1:2,000 - 1:10,000IHC: 1:250 - 1:1,000WB: 1:500 - 1:2,500Anti-Plasminogen has been tested by ELISA and western blot. This product is assayed against 1.0 µg of Plasminogen [Human Plasma] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethyl
Application Notes:
Anti-Plasminogen has been tested by ELISA and western blot. This product is assayed against 1.0 µg of Plasminogen [Human Plasma] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes
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