Anti-p53 (TP53) Mouse Monoclonal Antibody [Clone ID: OTI1D11], IgG1, Clone: [Clone: OTI1D11]

Catalog Number: BOB-M00001-5
Article Name: Anti-p53 (TP53) Mouse Monoclonal Antibody [Clone ID: OTI1D11], IgG1, Clone: [Clone: OTI1D11]
Biozol Catalog Number: BOB-M00001-5
Supplier Catalog Number: M00001-5
Alternative Catalog Number: BOB-M00001-5-100UL
Manufacturer: Boster Bio
Host: Mouse
Category: Antikörper
Application: FC, IF, IHC, WB
Species Reactivity: Human, Monkey, Mouse
Immunogen: Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.
Boster Bio TP53 mouse monoclonal antibody, clone OTI1D11 (formerly 1D11). Catalog M00001-5. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse.
Clonality: Monoclonal
Concentration: 0.98 mg/ml
Clone Designation: [Clone: OTI1D11]
Isotype: IgG1
UniProt: P04637
Buffer: PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation me
Application Dilute: WB 1:500~2000IHC 1:150IF 1:100Flow Cytometry 1:100
HEK293T cells transfected with either TP53 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TP53 antibody (M00001-5)
Flow cytometric Analysis of Hela cells
Anti-TP53 mouse monoclonal antibody (M00001-5) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.