E.coli-derived human Caveolin-1 recombinant protein (Position: G4-I178). Human Caveolin-1 shares 95% and 94% amino acid (aa) sequence identity with mouse and rat Caveolin-1, respectively.
Boster Bio Anti-Caveolin-1/CAV1 Antibody Picoband (monoclonal, 12C7) catalog M00179-1. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Monoclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). Caveolin-1/CAV1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1021) with DAB as the chromogen.
IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). Caveolin-1/CAV1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1021) with DAB as the chromogen.
IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). Caveolin-1/CAV1 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1021) with DAB as the chromogen.
IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). Caveolin-1/CAV1 was detected in paraffin-embedded section of human intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1021) with DAB as the chromogen.
IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV
Western blot analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human placenta tissue lysates,Lane 4: human U-87 whole cell lysates,Lane 5: human PC-3 whole cell lysates,Lane 6: human U20S whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Caveolin-1/CAV1 antigen affinity purified monoclonal antibody (Catalog M00179-1) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1001) with Tanon 5200 system. A specific band was detected for Caveolin-1/CAV1 at approximately 22KD. The expected band size for Caveolin-1/CAV1 is at 20KD.
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