Boster Bio Anti-BRG1 Rabbit Monoclonal Antibody catalog M00223. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the con
Purity:
Affinity-chromatography
Form:
Liquid
Target:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
Application Dilute:
WB 1:1000-5000IHC 1:50-200ICC/IF 1:50-200IP 1:20
IHC analysis of SMARCA4/BRG1 using anti-SMARCA4/BRG1 antibody (M00223). SMARCA4/BRG1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-SMARCA4/BRG1 Antibody (M00223) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of SMARCA4/BRG1 using anti-SMARCA4/BRG1 antibody (M00223). SMARCA4/BRG1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-SMARCA4/BRG1 Antibody (M00223) overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog SV0002) with DAB as the chromogen.
IHC analysis of SMARCA4/BRG1 using anti-SMARCA4/BRG1 antibody (M00223). SMARCA4/BRG1 w
Western blot analysis of SMARCA4/BRG1 using anti-SMARCA4/BRG1 antibody (M00223). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human HEL whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human PC-3 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCA4/BRG1 antigen affinity purified monoclonal antibody (M00223) at 1: 5000 overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog AR1196-200) with Tanon 5200 system. A specific band was detected for SMARCA4/BRG1 at approximately 220 kDa. The expected band size for SMARCA4/BRG1 is at 185 kDa.
Western blot analysis of SMARCA4/BRG1 using anti-SMARCA4/BRG1 antibody (M00223). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates,Lane 2: human HEL whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: rat C6 whole cell lysates,Lane 5: mouse RAW264.7 whole cell lysates,Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCA4/BRG1 antigen affinity purified monoclonal antibody (M00223) at 1: 5000 overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog AR1196-200) with Tanon 5200 system. A specific band was detected for SMARCA4/BRG1 at approximately 220 kDa. The expected band size for SMARCA4/BRG1 is at 185 kDa.
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