Anti-CD147 (BSG) Mouse Monoclonal Antibody [Clone ID: OTI9H5], IgG2b, Clone: [Clone: OTI9H5], Unconjugated

Product Overview

• Article Name: Anti-CD147 (BSG) Mouse Monoclonal Antibody [Clone ID: OTI9H5], IgG2b, Clone: [Clone: OTI9H5], Unconjugated
• Biozol Catalog Number: BOB-M00248-2
• Supplier Catalog Number: M00248-2
• Alternative Catalog Number: BOB-M00248-2-100UL
• Manufacturer: Boster Bio
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Canine, Human, Monkey, Rat
• Immunogen: Full length human recombinant protein of human BSG (NP_001719) produced in HEK293T cell.
• Conjugation: Unconjugated

Product Description

Boster Bio Anti-BSG (CD147) mouse monoclonal antibody, clone OTI9H5 (formerly 9H5). Catalog M00248-2. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Rat, Dog.

Product Properties

• Clonality: Monoclonal
• Concentration: 0.64 mg/ml
• Clone Designation: [Clone: OTI9H5]
• Isotype: IgG2b
• UniProt: P35613
• Buffer: PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation me

Application Notes

• Application Dilute: WB 1:1500~3000IHC 1:50IF 1:100Flow Cytometry 1:100

Images

HEK293T cells transfected with either BSG (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BSG antibody (M00248-2)

Anti-BSG mouse monoclonal antibody (M00248-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG.

Immunofluorescent staining of HT29 cells using anti-BSG mouse monoclonal antibody (M00248-2).

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BSG monoclonal antibody at 1:500 (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid gland, 9: colon,10: spleen).

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BSG monoclonal antibody.

Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and BSG-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-BSG monoclonal antibody M00248-2 (1:2000 ). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.