Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the con
Purity:
Affinity-chromatography
Form:
Liquid
Target:
Poliovirus receptor
Application Dilute:
WB 1:500-2000IP 1:40
Western blot analysis of CD155/PVR using anti-CD155/PVR antibody (M00664). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human U251 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: human U2OS whole cell lysates,Lane 6: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD155/PVR antigen affinity purified monoclonal antibody (M00664) at 1: 500 overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog AR1196-200) with Tanon 5200 system. A specific band was detected for CD155/PVR at approximately 70-80 kDa. The expected band size for CD155/PVR is at 45 kDa.
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