E. coli-derived human CDC45L recombinant protein (Position: E166-A431).
Alternative Names:
CDC45, CDC45L, CDC45L2, PORC PI 1
Boster Bio Anti-CDC45L Picoband Antibody (monoclonal, 6H6) catalog M01367-2. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Clonality:
Monoclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Purity:
Immunogen affinity purified.
Form:
Lyophilized
Target:
Cell division control protein 45 homolog
Application Dilute:
Western blot, 0.25-0.5µg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3µg/1x106 cells, Human
IHC analysis of CDC45L using anti-CDC45L antibody (M01367-2). CDC45L was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-CDC45L Antibody (M01367-2) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1021) with DAB as the chromogen.
IHC analysis of CDC45L using anti-CDC45L antibody (M01367-2). CDC45L was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-CDC45L Antibody (M01367-2) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1021) with DAB as the chromogen.
IHC analysis of CDC45L using anti-CDC45L antibody (M01367-2). CDC45L was detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-CDC45L Antibody (M01367-2) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1021) with DAB as the chromogen.
IHC analysis of CDC45L using anti-CDC45L antibody (M01367-2). CDC45L was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-CDC45L Antibody (M01367-2) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog SA1021) with DAB as the chromogen.
IHC analysis of CDC45L using anti-CDC45L antibody (M01367-2). CDC45L was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated wi
Western blot analysis of CDC45L using anti-CDC45L antibody (M01367-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CDC45L antigen affinity purified monoclonal antibody (Catalog M01367-2) at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1001) with Tanon 5200 system. A specific band was detected for CDC45L at approximately 66KD. The expected band size for CDC45L is at 66KD.
* VAT and and shipping costs not included. Errors and price changes excepted
