The TNF-alpha Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the TNF-alpha promoter. Tumor necrosis factor-alpha (TNF-alpha) is one of the major proinflammatory cytokines and can induce systemic inflammation, apoptotic cell death, sepsis and cachexia. Dysregulation of TNF-alpha induction is often involved in various human diseases including inflammatory bowel disease, cancer and Alzheimers disease. The TNF-alpha induction by lipopolysaccharide (LPS), the Toll-like receptor 4 (TLR4) ligand, is shown in Figure 1.
Buffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Dilute:
<h2>Application:</h2>Monitor the TNF-alpha induction activity. Screen for activators or inhibitors of the TNF-alpha signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medium supplemen
Fig-1: Induction of TNF-alpha promoter activity by LPS in TNF-alpha RAW264.7 cells.
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