TNF-beta Luciferase Reporter-RAW264.7 Cell Line

Product Overview

• Article Name: TNF-beta Luciferase Reporter-RAW264.7 Cell Line
• Biozol Catalog Number: BOB-RC1008
• Supplier Catalog Number: RC1008
• Alternative Catalog Number: BOB-RC1008-1VIAL
• Manufacturer: Boster Bio
• Category: Zellen/Zellkultur
• Application: FA

Product Description

The TNF-beta Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the TNF-beta promoter. Tumor necrosis factor-beta (TNF-beta) one of the major proinflammatory cytokines, which is produced predominantly by mitogen-stimulated T lymphocytes and leukocytes. TNF-beta is involved in the regulation of various biological processes such as cell proliferation, differentiation and apoptosis. TNF-beta gene polymorphisms are also significantly associated with disease susceptibility and phenotype in patients with psoriatic arthritis. The TNF-beta induction by lipopolysaccharide (LPS), the Toll-like receptor 4 (TLR4) ligand, is shown in Figure 1.

Product Properties

• Buffer: Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.

Application Notes

• Application Dilute: <h2>Application:</h2> Monitor the TNF-beta induction activity.Screen for activators or inhibitors of the TNF-beta signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medium supplemente

Images

Fig-1: Induction of TNF-beta promoter activity by LPS in TNF-beta RAW264.7 cells.