NFAT Luciferase Reporter-RAW264.7 Cell Line

Product Overview

• Article Name: NFAT Luciferase Reporter-RAW264.7 Cell Line
• Biozol Catalog Number: BOB-RC1011
• Supplier Catalog Number: RC1011
• Alternative Catalog Number: BOB-RC1011-1VIAL
• Manufacturer: Boster Bio
• Category: Zellen/Zellkultur
• Application: FA

Product Description

The NFAT Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Nuclear Factor of Activated T-cells (NFAT) response element, so that the cell line is designed to measure the transcriptional activity of NFAT. NFAT is a transcription factor originally found in activated T lymphocytes, and is now known to regulate not only T cell activation and differentiation but also the function of other immune cells including dendritic cells, B cells and megakaryocytes. The NFAT induction by calcium ionophore A23187 is shown in Figure 1.

Product Properties

• Buffer: Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.

Application Notes

• Application Dilute: <h2>Application:</h2>Monitor the NFAT signaling pathway activity.Screen for activators or inhibitors of the NFAT signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medium supplemented

Images

Fig-1: Induction of NFAT activity by calcium ionophore A23187 in NFAT RAW264.7 cells.