TLR3/ISRE Luciferase Reporter-HEK293 Cell Line

Product Overview

• Article Name: TLR3/ISRE Luciferase Reporter-HEK293 Cell Line
• Biozol Catalog Number: BOB-RC1023
• Supplier Catalog Number: RC1023
• Alternative Catalog Number: BOB-RC1023-1VIAL
• Manufacturer: Boster Bio
• Category: Zellen/Zellkultur
• Application: FA

Product Description

The TLR3/ISRE Luciferase cell line is a stably transfected HEK 293 cell line which expresses full-length human Toll-like receptor 3 (TLR3) and Renilla luciferase reporter gene under the transcriptional control of the interferon stimulated response element (ISRE). Functional activity of the cell line has been validated by TLR3 ligand assay (Figure 1), in which upon activation by poly (I:C), TLR3 quickly initiates TRIF-dependent signaling pathway and mediates nuclear translocation of IRF3 that induces ISRE activity.The TLR3/ISRE activation by poly (I:C) is shown in Figure 1.

Product Properties

• Buffer: Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.

Application Notes

• Application Dilute: <h2>Application:</h2>Monitor the TLR3 signaling pathway activity.Screen for activators or inhibitors of the TLR3 signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medium supplemented

Images

Fig-1: Induction of TLR3 activity by poly(I:C) in TLR3/ISRE HEK293 cells.