p53 Luciferase Reporter-HeLa Cell Line

Product Overview

• Article Name: p53 Luciferase Reporter-HeLa Cell Line
• Biozol Catalog Number: BOB-RC1026
• Supplier Catalog Number: RC1026
• Alternative Catalog Number: BOB-RC1026-1VIAL
• Manufacturer: Boster Bio
• Category: Zellen/Zellkultur
• Application: FA

Product Description

The p53 Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the p53 response element. p53 is a tumor suppressor that plays a crucial role in apoptosis and anticancer mechanisms. p53 reporter system is designed to monitor the p53-mediated signaling pathways.The p53 induction by doxorubicin is shown in Figure 1.

Product Properties

• Buffer: Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.

Application Notes

• Application Dilute: <h2>Application:</h2>Monitor the p53 induction activity.Screen for activators or inhibitors of the p53 signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medium supplemented with 10%

Images

Fig-1: Induction of p53 activity by doxorubicin in p53 HeLa cells.