The TLR2/NF-kB Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses full-length human Toll-like receptor 2 (TLR2) and Renilla luciferase reporter gene under the transcriptional control of the NF-kB response element. TLR2 is one of the key innate immune receptors. Functional activity of the cell line has been validated by TLR2 ligand assay, in which upon activation by Pam3CSK4, TLR2 quickly initiates downstream signaling pathway and mediates nuclear translocation of NF-kB (Figure 1).
Buffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Dilute:
<h2>Application:</h2> Monitor the TLR2 signaling pathway activity. Screen for activators or inhibitors of the TLR2 signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medium supplement
Fig-1: Induction of TLR2 activity by Pam3CSK4 in TLR2/NF-kB HEK293 cells.
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