The TLR9/NF-kB Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses full-length human Toll-like receptor 9 (TLR9) and Renilla luciferase reporter gene under the transcriptional control of the NF-kB response element. TLR9 is one of the key innate immune receptors. Functional activity of the cell line has been validated by TLR9 ligand assay, in which upon activation by CpG, TLR9 quickly initiates downstream signaling pathway and mediates nuclear translocation of NF-kB (Figure 1).
Buffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Dilute:
<h2>Application:</h2>Monitor the TLR9 signaling pathway activity.Screen for activators or inhibitors of the TLR9 signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medium supplemented
Fig.1: Induction of TLR9 activity by CpG in TLR9/NF-kB HEK293 cells.
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