The SRF-RE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the serum response factor-response element (SRF-RE). The SRF-RE reporter cell line can be used for studying GPCR-linked RhoA signaling pathways as well as screening of agonists, antagonists or signaling inhibitors related with the RhoA signaling pathways. Functional activity of the cell line has been validated by serum stimulation assay (Figure 1).
Buffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Dilute:
<h2>Application:</h2>Monitor the GPCR-linked RhoA signaling pathway.Screen for activators or inhibitors of the GPCR-linked RhoA signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medi
Fig-1: Induction of SRF-RE activity by FBS in SRF-RE HEK293 cells.
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