The MRE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the metal response element (MRE). MRE is targeted by MRE-binding transcription factor-1 (MTF-1) which is a zinc finger transcription factor and plays a major role in induction of metallothionein gene expression in response to cellular stress caused by heavy metals such as zinc and cadmium. The MRE induction by ZnSO4 is shown in Figure 1.
Buffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Dilute:
<h2>Application:</h2> Monitor MTF-1 transcriptional activity. Screen for activators or inhibitors of the MTF-1 signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medium supplemented w
Fig-1: Induction of MRE activity by zinc sulfate in MRE HEK293 cells.
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