ISRE Luciferase Reporter-RAW264.7 Cell Line

Product Overview

• Article Name: ISRE Luciferase Reporter-RAW264.7 Cell Line
• Biozol Catalog Number: BOB-RC1041
• Supplier Catalog Number: RC1041
• Alternative Catalog Number: BOB-RC1041-1VIAL
• Manufacturer: Boster Bio
• Category: Zellen/Zellkultur
• Application: FA

Product Description

The ISRE Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Interferon-Stimulated Response Element (ISRE), so that the cell line is designed to monitor the JAK/STAT signaling pathway activity. This cell line can be activated by type I IFNs as well as certain Toll like receptor ligands capable of induction of IRFs such as TLR3 ligand-poly (I:C). Functional activity of the cell line has been validated by poly (I:C) (Figure 1).

Product Properties

• Buffer: Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.

Application Notes

• Application Dilute: <h2>Application:</h2> Monitor the JAK/STAT signaling pathway activity.Screen for activators or inhibitors of the JAK/STAT signaling pathway. <h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using DMEM medium sup

Images

Fig-1: Induction of ISRE activity by poly(I:C) in ISRE RAW264.7 cells.