The NF-kB Luciferase Reporter cell line is a stably transfected Jurkat T cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the NF-kB response element. NF-kB is a key transcription factor that is involved in immune and inflammatory responses, developmental processes, cellular growth and apoptosis. The NF-kB induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1.
Buffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Dilute:
<h2>Application:</h2>Monitor the NF-kB signaling pathway activity.Screen for activators or inhibitors of the NF-kB signaling pathway. <h2>Culture conditions:</h2> Cells should be grown at 37C with 5% CO2 using RPMI medium supplemen
Fig-1: Induction of NF-kB activity by PMA in NF-kB Jurkat T cells.
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