The FOXP3 Luciferase Reporter cell line is a stably transfected Jurkat T cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Forkhead box P3 (FOXP3) promoter. As a member of the forkhead transcription factor family, FOXP3 is a key transcription factor that functions in the development and function of regulatory T cells. Functional activity of the cell line has been validated by phorbol 12-myristate 13-acetate (PMA) in the presence of ionomycin (Figure 1).
Buffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Dilute:
<h2>Application:</h2>Monitor the FOXP3 induction activity.Screen for activators or inhibitors of FOXP3 indction. <h2>Culture conditions:</h2> Cells should be grown at 37C with 5% CO2 using RPMI medium supplemented with 10% FBS, 1 m
Fig-1: Induction of NF-kB activity by PMA in FOXP3 Jurkat T cells.
* VAT and and shipping costs not included. Errors and price changes excepted
