EvaGreen Dye, 2000X in DMSO is offered for customers with specialized applications requiring a concentrated dye solution. Note: for most qPCR applications, we recommend our ready-to-use Forget-Me-Not(TM) EvaGreen qPCR Master Mixes or EvaGreen Dye, 20X in water. EvaGreen dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer. The unique properties of EvaGreen dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR Green I, EvaGreen dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen dye can be used at a much higher dye concentration than SYBR Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis. EvaGreen dye and applications are covered under granted and pending US and international patents.
Source:
Synthetic
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