Biotin-16-dUTP, Lyophilized Powder, CAS [[136632-31-0]]
Biozol Catalog Number:
BOT-40022-1
Supplier Catalog Number:
40022-1
Alternative Catalog Number:
BOT-40022-1
Manufacturer:
Biotium
Category:
Biochemikalien
Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3 end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies. The number 16 is the number of atoms in the linker between biotin and dUTP. Biotium also offers biotin-11-dUTP and biotin-20-dUTP. The length of the linker affects the incorporation efficiency of the biotin-dUTP probe into DNA using DNA polymerases, and it also affects biotin/avidin or biotin/streptavidin. In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. On the other hand, a longer linker should facilitate interaction between biotin and avidin or streptavidin. Lyophilized solid suitable for long term storage, contains lyophilized TE buffer Store at -20C C32H48N7O18P3Sli4 MW: 971.5 Biotin-16-dUTP is also available as a 1 mM solution in pH 7.5 Tris-HCl buffer.
Source:
Synthetic
CAS Number:
[136632-31-0]
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