DAPI (4,6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Because of the potential toxicity of DAPI, we offer DAPI dilactate dissolved in H2O as a safer alternative to weighing out the powdery form. We also offer DAPI dilactate solid, which is more water soluble than the dihydrochloride form of the dye. Classic blue nuclear counterstain for fixed cells Can be used at higher concentrations to stain live cells &lamba,Ex/&lamba,Em (with DNA) = 358/461 nm Yellow solution Store at 4C and protect from light C22H27N5O6 MW: 457.49
Source:
Synthetic
CAS Number:
[28718-91-4]
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