Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258. Either dye can be used to stain live or fixed cells in buffer or medium at 1 ug/mL with no wash step. Biotium also offers Hoechst 33342, 10 mg/mL in H2O. &lamba,Ex/&lamba,Em (with DNA) = 350/461 nm Pale yellow solid soluble in water up to 2% (w/v) Store at 4C and protect from light C27H31Cl3N6O.3H2O MW: 615.98 [23491-52-3]
Source:
Synthetic
CAS Number:
[23491-52-3]
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