Fura-2, pentapotassium salt is a ratiometric fluorescent dye that binds to free intracellular calcium. It is membrane-impermeant and may be loaded into cells via microinjection or scrape loading. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2-free) to 335 nm (Ca2-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. Light yellow solid soluble in DMSO Store at 4C and protect from light, especially in solution Kd: 145 nM (22C in pH 7.2 buffer) &lamba, Ex/&lamba,Em (no Ca2) = 363/512 nm, &lamba, Ex/&lamba, Em (high Ca2) = 335/505 nm epsilon (363 nm, no Ca2) = 27,000 M-1cm-1, epsilon (335 nm, high Ca2) = 35,000 M-1cm-1 C29H22K5N3O14 MW: 832 [113694-64-7]
Source:
Synthetic
CAS Number:
[113694-64-7]
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