5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator. Its excitation spectrum and fluorescence response to pH are similar to those of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading. &lamba,Ex/&lamba,Em (pH 9) = 492/514 nm epsilon (pH 9) = 82,000 Yellow orange solid soluble in pH>6 water Store at 4C and protect from light, especially in solution C21H12O7 MW: 376 [72088-94-9]
Source:
Synthetic
CAS Number:
[72088-94-9]
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