Biotin-4-fluorescein (N-(5-aminopentyl)biotinamide, trifluoroacetic acid salt) has been reported to be a superior fluorescent biotin derivative for accurate measurement of avidin and streptavidin in crude biological samples. Binding of biotin to avidin and streptavidin is high affinity, fast and noncooperative. &lamba,Ex/&lamba, Em = 494/523 nm Yellow solid soluble in DMSO, or pH>7 buffers Store at 4C and protect from light C33H32N4O8S MW: 644.7
Source:
Synthetic
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