Bovine, Canine, Ferret, Hamster, Human, Mouse, Porcine, Rat, Sheep
Immunogen:
Cytoskeletal preparation from HeLa cells
Conjugation:
CF568
Alternative Names:
CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8
This antibody reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology formalin-fixed 0.5-1.0 ug/mL|For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/mL PBS, 15 min at RT|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 0.5-1.0 ug/mL|Western blotting 0.5-1.0 ug/mL|Does not react with chicken , or Xenopus|Optimal dilution for a specific application should be determined by user
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