This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Clonality:
Monoclonal
Concentration:
0.1 mg/mL
Clone Designation:
[BTN/2032R]
Molecular Weight:
244Da
UniProt:
Not Applicable
Buffer:
PBS, 0.1% rBSA, 0.05% azide
Source:
Animal
Application Notes:
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow cytometry: 0.5-1 ug/million cells|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL for 30 minutes at RT|In situ Nucleic Acid Hybridization|No special pretreatment is required for the immunohistochemical staining of formalin-fixed paraffin-embedded tissues.|Western blotting|Optimal dilution for a specific application should be determined by user
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