Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) (KRTH/1576R), Biotin conjugate, 0.1mg/mL, Clone: [KRTH/1576R], Rabbit, Monoclonal

Product Overview

• Article Name: Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) (KRTH/1576R), Biotin conjugate, 0.1mg/mL, Clone: [KRTH/1576R], Rabbit, Monoclonal
• Biozol Catalog Number: BOT-BNCB1576-100
• Supplier Catalog Number: BNCB1576-100
• Alternative Catalog Number: BOT-BNCB1576-100-100UL
• Manufacturer: Biotium
• Host: Rabbit
• Category: Antikörper
• Application: IHC
• Species Reactivity: Human
• Immunogen: Recombinant full-length human KRT76 protein
• Conjugation: Biotin
• Alternative Names: KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9

Product Description

This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Properties

• Clonality: Monoclonal
• Concentration: 0.1 mg/mL
• Clone Designation: [KRTH/1576R]
• Molecular Weight: 52-67 kDa
• UniProt: Q01546
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Source: Animal

Application Notes

• Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.25-0.5 ug/mL|Immunofluorescence 1-2 ug/mL Western blotting 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user