Cytochrome C (Mitochondrial Marker)(6H2.B4), 0.2mg/mL, Clone: [6H2.B4], Mouse, Monoclonal
Biozol Catalog Number:
BOT-BNUB0184-100
Supplier Catalog Number:
BNUB0184-100
Alternative Catalog Number:
BOT-BNUB0184-100-100UL
Manufacturer:
Biotium
Host:
Mouse
Category:
Antikörper
Application:
FC, IF
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rat full-length cytochrome c protein
Alternative Names:
CYC, CYCS, HCS, THC4
Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow cytometry: 0.5-1 ug/million cells|Immunofluorescence: 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
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