IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker), 0.2mg/mL, Rabbit

Catalog Number: BOT-BNUB0728-500
Article Name: IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker), 0.2mg/mL, Rabbit
Biozol Catalog Number: BOT-BNUB0728-500
Supplier Catalog Number: BNUB0728-500
Alternative Catalog Number: BOT-BNUB0728-500-500UL
Manufacturer: Biotium
Host: Rabbit
Category: Antikörper
Application: IHC
Species Reactivity: Human
Immunogen: Purified human Ig Gamma Chain
Alternative Names: G1m Marker, G2m Marker, G3m Marker, G4m Marker, HDC, Heavy Chain Disease Protein, Human Immunglobulin G, Ig gamma1/2/3/4 Chain C Region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobulin Heavy Constant 1/2/3/4
This antibody recognizes a protein of 75 kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Concentration: 0.2 mg/mL
Molecular Weight: 75 kDa
UniProt: P01861, P01860, P01857, P01859
Buffer: PBS, 0.05% BSA, 0.05% azide
Source: Animal
Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user