Human IgM Immunoglobulin(DA4-4), 0.2mg/mL, Clone: [DA4-4], Mouse, Monoclonal
Biozol Catalog Number:
BOT-BNUB0759-500
Supplier Catalog Number:
BNUB0759-500
Alternative Catalog Number:
BOT-BNUB0759-500-500UL
Manufacturer:
Biotium
Host:
Mouse
Category:
Antikörper
Application:
IHC
Species Reactivity:
Human
Immunogen:
Heavy chain of human IgM
Alternative Names:
AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region
Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
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