Bromodeoxyuridine (BrdU) is a synthetic nucleoside thymidine analog. It is commonly used to detect proliferation in cells. In cells cultured in the presence of BrdU, BrdU will incorporate into newly synthesized DNA during S-phase. Antibodies specific for BrdU can then be used to detect replicated DNA. This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL).
Clonality:
Monoclonal
Concentration:
0.2 mg/mL
Clone Designation:
[BRD/1539R]
Molecular Weight:
307Da
UniProt:
Not Applicable
Buffer:
PBS, 0.05% BSA, 0.05% azide
Source:
Animal
Application Notes:
For coating for ELISA, order Ab without BSA|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
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