Cytochrome C (Mitochondrial Marker)(rCYCS/1010), 0.2mg/mL, Clone: [rCYCS/1010], Mouse, Monoclonal
Biozol Catalog Number:
BOT-BNUB3641-100
Supplier Catalog Number:
BNUB3641-100
Alternative Catalog Number:
BOT-BNUB3641-100-100UL
Manufacturer:
Biotium
Host:
Mouse
Category:
Antikörper
Application:
FC, IHC, WB
Species Reactivity:
Aves, Canine, Drosophila, Equine, Frog, Human, Mouse, Rat
Immunogen:
Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
Alternative Names:
CYC, CYCS, HCS, THC4
Recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i. e. cytochrome in the cytosol without heme attached) and holocytochrome (i. e cytochrome in the mitochondria with heme attached). Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
