Bovine Serum Albumin (BSA)(ALB/398), 1mg/mL, IgG1, Clone: [ALB/398], Mouse, Monoclonal
Biozol Catalog Number:
BOT-BNUM0398-50
Supplier Catalog Number:
BNUM0398-50
Alternative Catalog Number:
BOT-BNUM0398-50-50UL
Manufacturer:
Biotium
Host:
Mouse
Category:
Antikörper
Species Reactivity:
Bovine
Immunogen:
Purified bovine serum albumin
Alternative Names:
ALB, BSA, Growth inhibiting protein 20
This antibody reacts with a protein of ~66 kDa, identified as bovine serum albumin (BSA). It is a high affinity antibody and can be used for detection of traces of BSA. BSA is an abundant plasma protein in cows that is important for maintaining osmotic pressure in blood plasma for proper distribution of body fluids between intravascular compartments and body tissues. BSA is a common buffer component in immunoglobulin type assays due to good solubility characteristics for water, calcium, sodium, potassium, fatty acids, hormones and bilirubin. BSA makes up about half of the protein in plasma and represents the most stable and soluble protein in the plasma. It is a suitable reagent for laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling. BSA contains several lysines that are capable of reacting with conjugation sites of linkers, making it applicable as a carrier protein for antigenic compounds. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry|Does not react with human, rabbit, dog, or guinea pig, others not tested
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