Tenascin C (Stromal Marker For Epithelial Malignancy) (TNC/2981R), 1mg/mL, Clone: [TNC/2981R], Rabbit, Monoclonal

Product Overview

• Article Name: Tenascin C (Stromal Marker For Epithelial Malignancy) (TNC/2981R), 1mg/mL, Clone: [TNC/2981R], Rabbit, Monoclonal
• Biozol Catalog Number: BOT-BNUM2981-50
• Supplier Catalog Number: BNUM2981-50
• Alternative Catalog Number: BOT-BNUM2981-50-50UL
• Manufacturer: Biotium
• Host: Rabbit
• Category: Antikörper
• Application: IHC
• Species Reactivity: Human
• Immunogen: Recombinant full-length human TNC protein
• Alternative Names: Cytotactin, Glioma-associated-extracellular matrix antigen, GMEM, GP 150-225, Hexabrachion (HXB), JI, Myotendinous antigen, Neuronectin, TNC

Product Description

In Western blotting, this antibody reacts with two bands of ~MW of 210 kDa and 300 kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/mL
• Clone Designation: [TNC/2981R]
• Molecular Weight: 210 kDa and 300 kDa
• UniProt: P24821
• Buffer: PBS, no BSA, no azide
• Source: Animal

Application Notes

• Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Does not react with rat|Immunohistology (formalin): 2-4 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user