Tags Positive Control Whole Cell Lysate, Unconjugated
Biozol Catalog Number:
BSS-BS-41230P
Supplier Catalog Number:
bs-41230P
Alternative Catalog Number:
BSS-BS-41230P-250
Manufacturer:
Bioss
Category:
Zellen/Zellkultur
Application:
ELISA
Species Reactivity:
Other
Conjugation:
Unconjugated
This E.coli lysate contains a 41 kd recombinant protein expressing 11 commonly used Tags which are fused to the N-terminus of the protein in the order of: T7 HSV C-Myc VSV-g Glu-Glu V5 E-tag FLAG (DDDDK) S-tag HA 6x His GST Tag sequences: T7 = CMASMTGGQQMG HSV = QPELAPEDPEDC CMYC = EQKLISEEDLC VSV-g = YTDIEMNRLGK Glu-Glu = EYMPME V5 = GKPIPNPLLGLDST E-tag = GAPVPYPDPLEPR FLAG = DYKDDDDK HA = YPYDVPDYAC 6xHIS = HHHHHH Lyophilised: After the lyophilised pellet is dissolved in 250 ul SDS-gel sample buffer (1 mg/ml protein lysate) it can be used direct for sample loading and then stored at -20C. The sample can be reused several times. Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags.
Tag:
His
Buffer:
0.01M TBS(pH7.4)
Source:
Tags Positive Control Whole Cell Lysate protein is expressed in E.coli with His. It contains the amino acid sequence of .
Expression System:
E.coli
Purity:
> 95% as determined by SDS- PAGE
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