NLRC4 (Ser-533), Phosphospecific Antibody, Clone: [M549], Unconjugated, Rat, Monoclonal
Biozol Catalog Number:
BSS-BSM-70567T
Supplier Catalog Number:
bsm-70567T
Alternative Catalog Number:
BSS-BSM-70567T-100
Manufacturer:
Bioss
Host:
Rat
Category:
Antikörper
Application:
WB
Species Reactivity:
Human, Mouse, Rat
Conjugation:
Unconjugated
Alternative Names:
CARD12, CLAN1, IPAF, NLR family CARD domain-containing protein 4, NOD-like receptor 4
The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family is a diverse family of cytoplasmic innate immune receptors that are involved in recognition of pathogen-associated molecular patterns. NLRs are important for pathogen sensing, transcriptional activation of proinflammatory cytokines and activation of inflammatory caspases. NLRC4 (IPAF, CARD12) forms the inflammasome that responds to bacterial flagellin. This inflammasome is activated by NLRC4 oligomerization, NAIP protein binding, and activation of caspase-1 leading to pyroptosis. NLRC4 is phosphorylated on Ser-533 by PKCdelta following infection of macrophages with S. typhimurium. Mutant forms of NLRC4 demonstrate that an unphosphorylatable form (S533A) does not activate caspase-1 and pyroptosis in response to S. typhimurium, while a phosphomimetic NLRC4 (S533D) mutant causes rapid macrophage pyroptosis without infection. Thus, PKCdelta phosphorylation of NLRC4 (S533) may be a critical event in inflammasome activation and host innate immunity.
Clone M549 was generated from a phospho-NLRC4 (Ser-533) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding serine 533 in mouse NLRC4.
Target:
NLRC4 (Ser-533)
Application Dilute:
WB(1:300-5000)
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