MIP-1 alpha/CCL3, also known as LD78 alpha, is an inflammatory chemokine. MIP-1alpha belongs to the CCL chemokine family, and shares 68% homology with MIP-1beta. The mature form of MIP-1alpha contains 69 amino acids, exists as dimers in solution, and tends to undergo reversible aggregation. The receptors of MIP-1alphain vivo are mainly the G-protein coupled receptors CCR1 and CCR5. Upon stimulation by endogenous and exogenous agents such as Interleukin-1beta, Interferon-gamma, and lipoteichoic acid from Gram-positive bacteria, monocytes are able to secrete significant amounts of MIP-1alpha. MIP-1alpha augments the adhesions of T lymphocytes, monocytes, and neutrophils to vascular cell adhesion molecule 1. In addition, in wounds, MIP-1alpha chemo-attracts macrophages in order to accelerate the tissue repair process.Recombinant human MIP-1 alpha/CCL3 (rhMIP-1 alpha) produced in E.coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhMIP-1 alpha has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Molecular Weight:
7.8 kDa, observed by reducing SDS-PAGE.
Source:
Escherichia coli.
Purity:
> 95% as analyzed by SDS-PAGE and HPLC.
Form:
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence:
ASL AADTPTACCF SYTSRQIPQN FIADYFETSS QCSKPGVIFL TKRSRQVCAD PSEEWVQKYV SDLELSA
Formula:
Lyophilized after extensive dialysis against PBS.
Application Notes:
Reconstituted in ddH2O or PBS at 100 µg/ml.
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