Synthetic peptide, corresponding to amino acids 402-455 of Human Tubulin beta.
Conjugation:
HRP
Alternative Names:
Total tubulin beta, Tubulin beta
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form:
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Dilute:
WB: 1:10000 IHC: 1:100~1:1000
Application Notes:
Tubulin beta (4D9) mAb detects endogenous levels of beta-Tubulin protein.
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