PKC-pan (T495) polyclonal antibody, Unconjugated, Rabbit

Product Overview

• Article Name: PKC-pan (T495) polyclonal antibody, Unconjugated, Rabbit
• Biozol Catalog Number: BWT-BS1580
• Supplier Catalog Number: BS1580
• Alternative Catalog Number: BWT-BS1580-50UL,BWT-BS1580-100UL
• Manufacturer: Bioworld Technology
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide, corresponding to amino acids 470-520 of Human PKC-pan.
• Conjugation: Unconjugated
• Alternative Names: Protein kinase C alpha type, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA, Protein kinase C beta type, PKC-B, PKC-beta, PKCB, PRKCB1, PRKCB, Protein kinase C gamma type, PKC-gamma, PKCG, PRKCG, Protein kinase C delta type, Tyrosine-protein kinase PRKCD, nPKC-delta, Sphingosine-dependent protein kinase-1, SDK1, PRKCD, Protein kinase C epsilon type, nPKC-epsilon, PRKCE, PKCE, Protein kinase C zeta type, nPKC-zeta, PRKCZ, PKC2, PKC zeta, Protein kinase C theta type, nPKC-theta, PRKCQ, PRKCT

Product Description

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca2+.

Product Properties

• Molecular Weight: ~75 to 88 kDa
• UniProt: P17252
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Form: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Application Notes

• Application Dilute: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200
• Application Notes: PKC-pan (T495) polyclonal antibody detects endogenous levels of PKC alpha, beta I, beta II, gamma, delta, epsilon, eta, zeta and theta isoforms.