Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing and mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II). There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the majorconstituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm. hnRNP I, also designated polypyrimidine tract-binding protein (PTB) and its homolog hnRNP L bind to the 3 end of introns to modulate alternative splicing mechanisms of pre-mRNAs in normal cells and the translation of several viruses, including hepatitis C virus (HCV).
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Dilute:
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Application Notes:
hnRNP L (G58) polyclonal antibody detects endogenous levels of hnRNP L protein.
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