Synthetic phosphopeptide derived from human IkappaB-alpha around the phosphorylation site of Serine 32/Serine 36.
Conjugation:
Unconjugated
Alternative Names:
NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IkappaB proteins. Activation occurs via phosphorylation of IkappaBalpha at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-kappaB. IkappaBalpha phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IkappaB at these activating sites have been identified.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Application Dilute:
WB 1:500~1:1000
Application Notes:
IkappaB-alpha(Phospho-S32/S36) polyclonal antibody detects endogenous levels of IkappaB-alpha protein only when phosphorylated at Ser32/Ser36.
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