eIF4B (phospho-S422) polyclonal antibody, Unconjugated, Rabbit

Product Overview

• Article Name: eIF4B (phospho-S422) polyclonal antibody, Unconjugated, Rabbit
• Biozol Catalog Number: BWT-BS4311
• Supplier Catalog Number: BS4311
• Alternative Catalog Number: BWT-BS4311-50UL,BWT-BS4311-100UL
• Manufacturer: Bioworld Technology
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phosphopeptide derived from human eIF4B around the phosphorylation site of Serine 422.
• Conjugation: Unconjugated
• Alternative Names: Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B

Product Description

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.It is phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1.phosphorylation enhances the affinity of EIF4B for the EIF3 complex

Product Properties

• Molecular Weight: ~ 80 kDa
• UniProt: P23588
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Form: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Application Notes

• Application Dilute: WB: 1:500~1:1000 IHC: 1:50~1:200
• Application Notes: p-eIF4B (S422) polyclonal antibody detects endogenous levels of eIF4B protein only when phosphorylated at Ser422