Synthetic phosphopeptide derived from human SREBP-1 around the phosphorylation site of Serine 439
Conjugation:
Unconjugated
Alternative Names:
Sterol regulatory element-binding protein 1, SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regulatory element-binding transcription factor 1, Processed sterol regulatory element-binding protein 1, SREBF1, BHLHD1, SREBP1
Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5 flanking region, designated sterol regulatory element 1 (SRE-1). When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. SREBP-1 (also designated ADD1, for adipocyte determination and differentiation factor) is synthesized as a precursor that is attached to the nuclear envelope and endoplasmic reticulum. In sterol-depleted cells, the membrane-bound precursor is cleaved to generate a soluble NH2-terminal fragment that translocates to the nucleus to activate transcription. Sterols inhibit the cleavage of SREBP-1.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Application Dilute:
WB: 1:500~1:1000
Application Notes:
SREBP-1 (phospho-S439) polyclonal antibody detects endogenous levels of SREBP-1 protein only when phosphorylated at Ser439.
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