A synthetic peptide corresponding to residues in Human ROBO1
Conjugation:
Unconjugated
Alternative Names:
Roundabout homolog 1, Deleted in U twenty twenty, H-Robo-1, ROBO1, DUTT1
Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior . In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively . These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway . One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals . Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion . Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross . Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout . Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray .
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Dilute:
WB: 1:500~1:1000
Application Notes:
ROBO1 polyclonal antibody detects endogenous levels of ROBO1 protein.
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