Tubulin is a major cytoskeleton component that has five distinct forms, designated alpha, beta, gamma, delta and epsilon Tubulin. alpha and beta Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple beta Tubulin isoforms (beta1, beta2, beta3, beta4, beta5, beta6 and beta8) have been characterized and are expressed in mammalian tissues. beta1 and beta4 are present throughout the cytosol, beta2 is present in the nuclei and nucleoplasm, and beta3 is a neuron-specific cytoskeletal protein. gamma Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both delta Tubulin and epsilon Tubulin are associated with the centrosome. delta Tubulin is a homolog of the Chlamydomonas delta Tubulin Uni3 and is found in association with the centrioles, whereas epsilon Tubulin localizes to the pericentriolar material. epsilon Tubulin exhibits a cellcycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Application Dilute:
WB: 1:500~1:1000
Application Notes:
TUBA1A (phospho-Y272) polyclonal antibody detects endogenous levels of TUBA1A protein only when phosphorylated at Try272 .
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